《植物生理学报》 2012, 48(1): 85-89

药用植物华泽兰组织培养和快速繁殖

梁钻姬, 潘超美*, 赖珍珍, 郑芳昊, 夏静, 刘欣

广州中医药大学中药学院, 广州510006

通信作者：潘超美；E-mail: pancm@gzhtcm.edu.cn；Tel: 020-39358249

摘　要：

通过比较不同的外植体、植物生长调节剂种类和浓度配比、生根培养基类型以及生根苗的移栽基质, 建立华泽兰组织培养快速繁殖体系。结果表明, 外植体表面消毒以75%酒精预处理10 s, 再用0.1% HgCl2浸泡10 min, 以嫩茎节为外植体诱导效果最好。培养基MS+6-BA 1.00 mg·L-1+IBA 0.05 mg·L-1利于形成丛生芽, 用于继代增殖, 30 d的增殖系数为9.43;1/2MS+IBA 0.05~0.10 mg·L-1适宜诱导生根获得再生植株, 生根率100%; 生根苗适宜移栽于田园土中, 成活率93.3%。

关键词：华泽兰; 组织培养; 快速繁殖

收稿：2011-09-07   修定：2011-10-21

资助：广东省重点科技专项(2004B60302001)和湛江市农业局科技专项。

Tissue Culture and Rapid Propagation of Medicinal Plant Eupatorium chinense L.

LIANG Zuan-Ji, PAN Chao-Mei*, LAI Zhen-Zhen, ZHENG Fang-Hao, XIA Jing, LIU Xin

College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China

Corresponding author: PAN Chao-Mei; E-mail: pancm@gzhtcm.edu.cn; Tel: 020-39358249

Abstract:

The techniques of tissue culture and rapid propagation of Eupatorium chinense L. were studied by comparing with different explants, types and concentrations of plant growth regulartors, types of rooting medium and transplanting medium. The results showed that the most suitable procedure for sterilization was to soak explants into 0.1% HgCl2 solution for 10 min after pretreatment with 75% alcohol for 10 s. The tender stems were the best explants. MS+6-BA 1.00 mg·L-1+IBA 0.05 mg·L-1 could effectively induce fasciculate buds and be suitable for subculture and proliferation, the propagation coefficient was 9.43 every 30 days; the most optimum rooting medium was 1/2MS+IBA 0.05-0.10 mg·L-1, and the rooting rate was 100%. The rooting seedlings were effectively transplanted in glebe soil. The highest survival rate was 93.3%.

Key words: Eupatorium chinense; tissue culture; rapid propagation